The Inaugural Flatiron Institute Cryo-EM Conformational Heterogeneity Challenge.

ABSTRACT: Despite the rise of single particle cryo-electron microscopy (cryo-EM) as a premier method for resolving macromolecular structures at atomic resolution, methods to address molecular heterogeneity in vitrified samples have yet to reach maturity. With an increasing number of new methods to analyze the multitude of heterogeneous states captured in single particle images, a systematic approach to validation in this field is needed. With this motivation, we issued a challenge to the community to analyze two cryo-EM particle image sets of thyroglobulin that exhibit continuous conformational heterogeneity. The first dataset was experimental and the second was generated with a simulator, allowing control over the distribution of molecular structures and enabled direct comparison between participan

SUBMITTER: Astore MA 

PROVIDER: S-EPMC12637659 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature