Inducible Calling Cards: Developing Mouse Reagents for Experimentally Controlled Transposon Insertion <i>in vivo</i>.

ABSTRACT: The piggyBac transposase enables robust forward genetic screens in mice. Further, fusions of piggyBac with specific transcription factors (TFs) enables recovery of recoverable transposons (Calling Cards) for recording location of DNA binding events in vitro and in vivo. Such applications would be enhanced by engineering inducible transposases, such that timing of recording could be precisely controlled in vivo, as has been previously developed in vitro. Here, we tested two approaches for applying inducible Calling Cards (iCC) in the murine brain. We engineered knock-ins of inducible versions of two TFs: Jun, an immediate early gene that serves as a proxy for neural activity, and Sp1, a promiscuous binder of CpG unmethylated regions that indicates active promoter

SUBMITTER: Sarafinovska S 

PROVIDER: S-EPMC12637670 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature