Development and validation of ultra performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) method to quantify monotropein in blueberries.

ABSTRACT: Blueberries (Vaccinium species) are an economically important fruit crop rich in bioactive compounds like polyphenols and flavonoids. Interestingly, some blueberry cultivars also produce monotropein, which has bioactive properties, including anti-inflammatory and neuroprotective effects. However, methods to quantify monotropein in blueberries have not been optimized. To address this gap, an optimized analytical method for monotropein extraction and quantification using ultra performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) was developed. Different extraction strategies were compared, including variations in temperature, time, and ultrasonication treatments. Optimal extraction was achieved by heating samples to 60 °C for 15 mins in methanol. The method had high percen

SUBMITTER: Kaur I 

PROVIDER: S-EPMC12637891 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature