Single-cell RNA sequencing reveals the role of immunoinflammatory cells in the progression of renal tubulointerstitial fibrosis.

ABSTRACT: Renal tubulointerstitial fibrosis (TIF) is an independent risk factor for chronic kidney disease (CKD) progression and prognosis. It is known that immunoinflammatory cell infiltration plays a crucial role in TIF development and progression. However, what types of immunoinflammatory cells and by what means they promote TIF have not been fully clarified. In this study, mice models of unilateral ureteral obstruction (UUO) in which the left ureters were ligated for 3, 7, and 14 days, respectively, were used to simulate different levels of TIF severity. Single-cell RNA sequencing (scRNA-seq) was performed to characterize the immunoinflammatory cells in the kidneys of mice in each group. The results showed that the degree of renal pathological injury and expression level of fibrosis-related prot

SUBMITTER: Ye X 

PROVIDER: S-EPMC12637933 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature