The B169L protein of African swine fever virus functions as a viroporin that activates the calcium-mediated inflammasome.

ABSTRACT: African swine fever (ASF) is a highly contagious and often fatal viral disease caused by African swine fever virus (ASFV), which poses a significant economic burden on the global pig industry. ASFV infection triggers a robust production of proinflammatory cytokines, leading to severe inflammation that contributes significantly to the high mortality rate associated with ASF. However, the underlying mechanisms remain incompletely understood. Here, we identified the ASFV B169L protein (pB169L) as a viroporin that exerts dual functions in viral replication and proinflammatory responses. We demonstrated that pB169L formed oligomeric calcium (Ca2+)-permeable channels in vitro by bilayer lipid membrane assay. The ectopically expressed pB169L significantly altered Ca2+ homeostasis in cells and ind

SUBMITTER: Li J 

PROVIDER: S-EPMC12638030 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature