Secondary metabolites of Minutisphaera thailandensis and Hongkongmyces kokensis revealed by LC-QTOF-MS and their antimicrobial and cytotoxic activities.

ABSTRACT: The mycelial extracts of two novel fungal species, Minutisphaera thailandensis and Hongkongmyces kokensis, were investigated for their antimicrobial, cytotoxic, and secondary metabolite profiles to assess their potential as sources of bioactive natural products. LC-QTOF-MS profiling revealed that the mycelial extracts contained a greater diversity of metabolites compared to the culture supernatants. M. thailandensis demonstrated strong antibacterial activity against Bacillus subtilis (MIC = 62.25 µg/mL; inhibition zone = 20.12 ± 0.21 mm), Staphylococcus epidermidis (125.00 µg/mL; 19.32 ± 0.15 mm), and Staphylococcus aureus subsp. aureus (125.00 µg/mL; 19.08 ± 0.12 mm). H. kokensis was active against Pseudomonas aeruginosa (16.45 ± 0.28 mm) and Escherichia coli (16.87 ± 0.36 mm). Both fungi

SUBMITTER: Khruengsai S 

PROVIDER: S-EPMC12638296 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature