Design and evaluation of octopromycin-derived peptides as multifunctional antimicrobial agents against multidrug-resistant pathogens.

ABSTRACT: Octopromycin (OPM), an antimicrobial peptide from Octopus minor, shows potent activity against multidrug-resistant (MDR) Acinetobacter baumannii. Guided by the structure-activity relationship of the N-terminal domain OPM23, we designed short α-helical peptides with enhanced antimicrobial, anti-inflammatory, and antibiofilm properties while maintaining low toxicity. Antimicrobial activity was assessed by broth microdilution; protease and serum stability by HPLC. Mechanism of action was examined using membrane depolarization, NPN uptake, β-galactosidase (ONPG) hydrolysis, flow cytometry, and scanning electron microscopy. Anti-inflammatory effects were evaluated by ELISA, RT-PCR, and lipopolysaccharide (LPS) interaction assays, and biofilm inhibition/eradication was tested against MDR Pseudom

SUBMITTER: Ganbaatar B 

PROVIDER: S-EPMC12638300 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature