A yeast surface display platform for characterizing CAR T cell responses to cancer antigens.

ABSTRACT: Chimeric antigen receptor (CAR) T cells have become an established immunotherapy with promising results for the treatment of hematological malignancies. However, modulation of the targeted antigen's surface level in cancer cells affects the quality and safety of CAR-T cell therapy. Here we present an engineered yeast-based antigen system for simulation of cancer cells with precise regulation of surface-antigen densities, providing a tool for controlled activation of CAR T cells and systematic assessment of antigen density effects. This Synthetic Cellular Advanced Signal Adapter (SCASA) system uses G protein-coupled receptor signaling to control cancer antigen densities on the yeast surface and provides a customizable platform allowing selectable signal inputs and modular pathway engineerin

SUBMITTER: Deichmann M 

PROVIDER: S-EPMC12638309 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature