LETM2 regulates mitochondrial function and autophagy in diabetic foot ulcers: implications for oxidative stress and therapeutic targeting.

ABSTRACT: Background A major complication of diabetes, diabetic foot ulcers (DFU), has been linked to mitochondrial dysfunction and oxidative stress. Here, we performed analysis of RNA sequencing data from publicly available databases, including single-cell assays, to identify dysregulated genes associated with these processes in DFU and the specific cell types involved. Methods Analysis of transcriptome sequencing was performed on GSE134431 and GSE80178 datasets from the GEO database to identify differentially expressed mitochondrial-related genes (DE-MRGs) in DFU samples relative to controls. LASSO and SVM-RFE algorithms were used to identify critical genes, and functional enrichment analyses were conducted to identify associated pathways. Results were validated in a cohort of DFU samples and cont

SUBMITTER: Su N 

PROVIDER: S-EPMC12638387 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature