Sympathovagal crosstalk: Y2-receptor blockade enhances vagal effects which in turn reduce NPY levels via muscarinic receptor activation.

ABSTRACT:

Aims

Ventricular arrhythmias are associated with sympathoexcitation and increased co-transmitter neuropeptide Y (NPY) levels. Vagal nerve stimulation (VNS) has been reported to decrease release of norepinephrine, while NPY has been reported to decrease acetylcholine release ex vivo by binding Y2 receptors on parasympathetic nerves. We hypothesized that VNS reduces NPY levels via a muscarinic receptor (MR) mediated mechanism in vivo and that, in turn, blockade of pre-synaptic Y2R can further enhance the effects of VNS and decrease the effects of sympathoexcitation by increasing vagal tone.

Methods and results

Single-cell RNA sequencing of rat stellate ganglia and immunohistochemistry were performed and identified the M2 receptor as the predominant subtype on NPY-expressing sy

SUBMITTER: Jani NR 

PROVIDER: S-EPMC12638732 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature