ARMH2 is a cytosolic component of CatSper crucial for sperm function.

ABSTRACT: Sperm capacitation and fertilization are highly regulated by Ca²⁺ signaling. CatSper, a sperm-specific calcium channel, plays a crucial role in sperm hyperactivated motility and fertility by mediating Ca²⁺ influx into sperm. CatSper is the most complicated ion channel known, comprising the pore-forming CATSPER1-4 and multiple auxiliary subunits. However, our previous structural study of mouse CatSper suggests the presence of potential component(s) that remain to be identified. The identity and functional significance of the missing piece(s) of CatSper remain elusive. Here, by combining cryo-EM, mass spectrometry, AlphaFold structure prediction, and coevolutionary analysis, we identify armadillo-like helical domain containing 2 (ARMH2) as a cytosolic component of CatSp

SUBMITTER: Zhao Q 

PROVIDER: S-EPMC12638846 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature