A novel malignant mesothelioma organoids-T cell co-culture platform for personalized immunochemotherapy testing.

ABSTRACT: Malignant mesothelioma (MM) is a rare, aggressive cancer with poor outcomes due to its heterogeneity and therapy resistance. Patient-derived organoids (PDOs) have emerged as a promising tool for precision medicine, as they faithfully recapitulate key tumor characteristics. This study aimed to establish MM PDOs and develop a PDO-T cell co-culture system to evaluate their utility in guiding personalized MM treatment. MM PDOs were generated from malignant ascites, pleural effusions, fine-needle biopsies, and surgical tissues. Organoids were characterized using H&E staining, immunohistochemistry (IHC), and next-generation sequencing (NGS). PBMC-derived T cells were co-cultured with PDOs to assess immunotherapy and chemotherapy responses. Drug sensitivity testing was performed to evaluate treat

SUBMITTER: Liu Y 

PROVIDER: S-EPMC12638976 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature