Mapping of HOCl-oxidized RNA identifies abasic sites as major damage and oxidation product of oxo⁸G.

ABSTRACT: RNA oxidation is an important yet understudied process, partly because methods to localize oxidized residues in RNA are lacking. We introduce OAbSeq, a deep-sequencing approach that maps oxidized sites with high sensitivity by exploiting aniline-induced strand scission at noncanonical nucleosides to generate unique ligation-competent fragments utilized for library preparation. Applied to yeast RNA, OAbSeq detects widespread signals predominating at purines, especially at guanosines. Exogenous oxidation increased signal intensity but preserved the guanosine-dominated pattern. Parallel quantification of 8-oxoguanosine (oxo⁸G) and abasic sites revealed that abasic sites are more abundant than oxo⁸G following oxidative treatment in vitro and under physiological conditions

SUBMITTER: Weber M 

PROVIDER: S-EPMC12639000 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature