Revealing the complexity of the epicardial secretome.

ABSTRACT: The epicardium, an epithelial layer covering the heart, plays pivotal roles in embryonic heart development and responses to adult cardiac damage. Epicardial-secreted molecular agents are known to be involved in the regulation of these phenomena, but how this regulation occurs is poorly understood. In this study, we have investigated extracellular vesicle (EV) and extracellular matrix (ECM) components of the epicardial secretome using a continuous mouse embryonic epicardial-derived cell (EPIC) line. Epicardial-derived EVs were isolated using differential ultracentrifugation from EPIC cultured at 1% (EVs-H1%), 5% (EVs-H5%), and 21% oxygen (EVs-N). EVs protein content was determined by tandem mass tag (TMT) proteomic analysis. The results showed that epicardial-derived EVs cargo is sensitive

SUBMITTER: Oliveira CC 

PROVIDER: S-EPMC12639070 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature