Mechanism of cooperative strigolactone perception by the MAX2 ubiquitin ligase-receptor-substrate complex.

ABSTRACT: Strigolactones are plant hormones that regulate development and mediate interactions with soil organisms, including the germination of parasitic plants such as Striga hermonthica. Strigolactone perception by receptors initiates the degradation of transcriptional repressors via E3 ubiquitin ligases, but the mechanistic link between hormone binding and substrate ubiquitination has remained unclear. We determine cryogenic electron microscopy structures of the receptor-ligase-substrate complex, composed of Arabidopsis ASK1 and substrate, and Striga F-box and receptor proteins. Strigolactone hydrolysis by the receptor, which covalently retains the D-ring, is a prerequisite for complex formation. The substrate engages the complex through two domains, forming a dynamic interface that stabilises t

SUBMITTER: Vancea AI 

PROVIDER: S-EPMC12639087 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature