Chromosome-level genome assembly and annotation of the Triplophysa pappenheimi.

ABSTRACT: Triplophysa pappenheimi, an ecologically important species within the plateau freshwater ecosystem, predominantly inhabits the rapids of the upper Yellow River, its tributaries, and associated lakes, playing a crucial role in maintaining local aquatic biodiversity and serving as a model for plateau fish adaptation studies. In this study, we utilized a combination of Illumina short-read sequencing, PacBio HiFi long-read sequencing, and Hi-C technology to generate the first high-quality, chromosome-level genome assembly of T. pappenheimi. The assembled genome spans 626.33 Mb, organized into 25 pseudochromosomes, with a scaffold N50 of 24.41 Mb. We annotated 24,952 genes, representing 98.82% of the predicted protein-coding genes. In addition, we used GetOrganelle v1.7.7.1 to isolate and assem

SUBMITTER: Li Y 

PROVIDER: S-EPMC12639136 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature