Parkinson's associated protein DJ-1 regulates intercellular communication via extracellular vesicles in oxidative stress.

ABSTRACT: Mutations in DJ-1 cause autosomal recessive Parkinson's disease (PD). Several functions have been attributed to DJ-1, including a key role in the protection from oxidative stress. However, how this protein contributes to PD pathogenesis is still unclear. Recently, DJ-1 has been identified at higher concentrations in extracellular vesicles (EV) from biological fluids of PD patients, providing a link between EV and a protein associated with PD. In this study, EV were purified from the medium of control and rotenone-treated wild-type and DJ-1 KO differentiated SH-SY5Y cells. EV quantity was assessed using flow cytometry, and their proteomic cargo was analysed via mass spectrometry. We identified an altered EV response to rotenone in DJ-1 KO cells compared to wild-type. Mass spectrometry analy

SUBMITTER: Page T 

PROVIDER: S-EPMC12639138 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature