Hydrogel microcapsulation technology reduces the DMSO concentration required for stem cell cryopreservation.

ABSTRACT:

Introduction

Stem cell therapy is increasingly advancing towards clinical applications, with cryopreservation playing a critical role in cell transplantation. Previous studies have demonstrated that hydrogel microcapsules can enhance cell survival during cryopreservation. However, most research has focused on rapid freezing techniques or cryopreservation with high-concentration dimethyl sulfoxide (DMSO). This study aims to investigate the effects of hydrogel microcapsules on the viability, phenotype, and functionality of mesenchymal stem cells (MSCs) following cryopreservation with low-concentration DMSO. The objective is to develop a safer cryopreservation strategy for clinical stem cell applications.

Methods

In this study, We utilized five concentrations of DMSO (0 %, 1.0 

SUBMITTER: Tang Z 

PROVIDER: S-EPMC12639263 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature