Stage-specific antiviral roles of IFIH1, IRF7, and MAVS in the interferon-mediated response to DHAV-3 infection.

ABSTRACT: Duck hepatitis A virus genotype 3 (DHAV-3) is the predominant causative agent of duck viral hepatitis in East Asia, yet its mechanisms of immune evasion remain unclear. In this study, primary hepatocytes were collected at 6, 12, and 24 h post-infection (hpi). The results showed that overall IFN-I levels were significantly elevated after infection, with a particularly pronounced upregulation of IFN-β. Silencing of IFN-I expression markedly promoted DHAV-3 replication, indicating its distinct antiviral role. Furthermore, eight innate immune molecules (IFIH1, IRF1, IRF7, MAVS, RIG-I, TBK1, TLR3, and TLR4) related to IFN-I were investigated in primary duck liver cells using interference and overexpression assays. At 6 hpi, silencing IRF7 and TLR4 markedly promoted replication, while TLR3 inhib

SUBMITTER: Li H 

PROVIDER: S-EPMC12639309 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature