Integration analysis of transcriptomics and proteomics reveals molecular pathways of duck intestinal epithelial cells in response to oxidative stress.

ABSTRACT: Previous studies have shown that ducks undergo stress when transferred to a cage, and intestinal oxidative damage is induced. The intestinal mucosa is an important target of stress, and the terminal intestinal epithelial cells of the villi are the most strongly affected by oxidative stress. To investigate the molecular mechanism of oxidative damage in duck intestinal epithelial cells (dIECs), damage to dIECs was detected by inducing oxidative stress with different concentrations (0, 50, 100, and 200 μM) of hydrogen peroxide (H₂O₂) in primary dIECs, and molecular pathways were analyzed using combined transcriptomics and proteomics. The results revealed that oxidative stress significantly increased the reactive oxygen species (ROS) levels and apoptosis rate (P < 0.05), decreased antioxidant

SUBMITTER: He X 

PROVIDER: S-EPMC12639317 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature