Alcohol-related hepatitis induces a specific fibrosis profile through YAP activation in myofibroblasts.

ABSTRACT:

Background & aims

Yes-associated protein (YAP) impairs hepatocyte regeneration in alcohol-related hepatitis (AH), but its impact on fibrogenesis remains to be characterized. Our aim here was to describe fibrogenesis and investigate the impact of altered hepatocytes on fibrogenic mechanisms during AH.

Methods

Fibrosis, YAP and alpha smooth muscle actin (αSMA) distribution were assessed by immunostaining. Extracellular matrix (ECM) composition in severe AH (n = 22), alcohol-related cirrhosis without AH (Cirrh, n = 24), and healthy livers (CTRL, n = 15) was assessed by PCR, and bulk and spatial proteomics. The impact of organoids generated from AH and Cirrh livers on myofibroblasts was also evaluated. Cirrh organoids were transduced with constitutively active YAP (to mimic AH o

SUBMITTER: Wandji LCN 

PROVIDER: S-EPMC12639322 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature