Nitric oxide attenuates PI4P accumulation at the ER membrane to inhibit encephalomyocarditis virus replication selectively in β-cells.

ABSTRACT: Viral infection, particularly by members of the picornavirus family, has been associated with autoimmune diabetes (type 1 diabetes mellitus) onset. The encephalomyocarditis virus (EMCV) is a mouse-tropic member of the picornavirus family that stimulates innate immune activation, leading to the production of cytokines. In response to cytokines, β-cells express inducible nitric oxide (NO) synthase and produce low micromolar levels of the free radical, NO. We have previously shown that, because of its inhibitory action on mitochondrial oxidation and depletion of cellular ATP, NO selectively attenuates EMCV replication in, and lysis of, β-cells. In this study, we show that one mechanism by which NO inhibits EMCV replication is by attenuating the accumulation of phosphatidylinositol-4-phosphate

SUBMITTER: Gehant AL 

PROVIDER: S-EPMC12639437 | biostudies-literature | 2025 Oct

REPOSITORIES: biostudies-literature