Quantification of eight clinically relevant serum adrenal steroids in infants by UHPLC-APCI-MS/MS.

ABSTRACT:

Background and aims

There is growing interest in replacing steroid immunoassays with liquid chromatography-tandem mass spectrometry-based methods in many clinical and research applications. The aim of the present work is to develop and validate an ultra-high-performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS)-based method using atmospheric pressure chemical ionisation (APCI) to quantify cortisol, androstenedione, testosterone, pregnenolone, progesterone, 17-hydroxyprogesterone, 17-hydroxypregnenolone, and dehydroepiandrosterone in paediatric serum samples.

Methods

Sample preparation was performed by protein precipitation using 100 µL of sample. The analytical method was validated in accordance with FDA and EMA international guidelines. Commercial

SUBMITTER: Martinefski MR 

PROVIDER: S-EPMC12639463 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature