Development of a cost-effective LAMP method for screening and monitoring enteropathogenic <i>Yersinia</i> in environmental matrices.

ABSTRACT:

Background

Closely related bacterial pathogens, Yersinia pseudotuberculosis, Yersinia pestis (the plague agent), and Yersinia enterocolitica, reside in environmental ecosystems that may be the source of infection for animals and humans. Reliable field-deployable detection methods are essential for monitoring these pathogens in natural ecosystems.

Methods

A colorimetric loop-mediated isothermal amplification (LAMP) assay targeting the mglB gene (shared by Y. pseudotuberculosis and Y. pestis) and the chbG gene (specific to Y. enterocolitica) was developed. Specificity was evaluated using six Yersinia isolates, and sensitivity was determined from serial dilutions of Y. enterocolitica and Y. pseudotuberculo

SUBMITTER: Oumarou Hama H 

PROVIDER: S-EPMC12639587 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature