Diagnostic potential of GLP recombinant antigens in whole blood assays for Leishmania infantum infection.

ABSTRACT:

Background

The whole blood stimulation assay (WBA) is a valuable tool for detecting asymptomatic Leishmania infection and monitoring the treatment of visceral leishmaniasis (VL). This study sought to identify specific recombinant proteins to replace the nonspecific soluble Leishmania antigen in this assay, which could be useful for developing a standardized diagnostic test that complies with good manufacturing practice.

Methods

Employing a cell lymphoproliferative assay, we here assessed the behaviour of 11 recombinant antigens in 61 subjects who had either been successfully treated for or had spontaneously recovered from Leishmania infantum infection. We then selected those antigens showing significant differences in immune cell stimulation indices and cytokine secretion be

SUBMITTER: Ibarra-Meneses AV 

PROVIDER: S-EPMC12639648 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature