Development and clinical application of a rapid qPCR instrument featuring three independent temperature modules and a time-based algorithm for respiratory pathogen diagnosis.

ABSTRACT:

Background

Acute respiratory infections (ARIs) caused by viruses, bacteria, Mycoplasma, and other microorganisms rank among the most serious human health threats, and they typically manifest similar flu-like symptoms during the early stages of infection. The rapid and accurate diagnosis of pathogens causing ARIs is crucial for treating diseases and controlling pathogen transmission. Quantitative real-time polymerase chain reaction (qRT-PCR) serves as the primary detection method, yet current workflows are time-consuming and have limited multiplexing capacity; thus, they fail to meet on-site diagnostic requirements. Our aim is to develop a novel method that enhances heating and cooling rates, thereby significantly shortening PCR cycling time and enabling the on-site rapid detection

SUBMITTER: Wang Y 

PROVIDER: S-EPMC12639651 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature