Cellular Responses During Kidney Normothermic Machine Perfusion Reflect Graft Outcomes.

ABSTRACT:

Introduction

Kidneys donated after circulatory death and kidneys from older donors demonstrate increased susceptibility to ischemia reperfusion injury and immune attack after transplantation. However, there are currently no assessment tools that can reliably predict posttransplant outcome of donor kidneys. In this study, we investigated whether the cellular responses of kidneys during normothermic machine perfusion (NMP) are associated with posttransplant outcome.

Methods

Snap-frozen biopsies from 6 kidneys taken before and after 2-hour NMP were subjected to single nucleus RNA sequencing (snRNA seq). Biopsies from another 22 kidneys taken at the same time points were included for quantitative real-time PCR (qPCR) to confirm the snRNA seq data. Estimated glomerular filtration

SUBMITTER: Li S 

PROVIDER: S-EPMC12639818 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature