Differential increase in endocannabinoid levels at the plasma and intracellular membranes.

ABSTRACT: Endocannabinoids (eCBs) modulate the activity of proteins expressed at the plasma and intracellular membranes. Nothing is known about the dynamic changes in eCB levels in these subcellular compartments. We leveraged the eCB sensor, GRAB_eCB2.0, to establish the stimulus-induced increases in the eCB, 2-arachidonoyl glycerol (2-AG), at the plasma and intracellular membranes of undifferentiated Neuro2a cells in culture. Activating G protein-coupled B₂ receptors with bradykinin increased 2-AG levels at both the plasma and intracellular membranes within ≈5 and ≈15 s, respectively. By contrast, the activation of G proteins by the small peptide mastoparan and the ensuing opening of plasma membrane calcium channels increased 2-AG levels in plasma membrane within ≈1-2 s and in

SUBMITTER: Singh S 

PROVIDER: S-EPMC12639868 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature