Biological nanoparticles from Brucella abortus_{ΔeipB∆perΔwadC} elicit protective immunity against brucellosis.

ABSTRACT: Brucellosis, caused by bacteria of the genus Brucella, is a globally prevalent zoonotic disease that poses serious risks to both animal and human health. Brucella abortus releases the biological nanoparticles (outer-membrane-vesicles, OMVs), which contribute to chronic infection by promoting host invasion and immune evasion. In this study, we performed a detailed characterization of OMVs derived from the wild-type B. abortus strain. These OMVs enhanced bacterial invasion and intracellular survival in macrophages ex vivo and increased pathogenicity in mice by modulating macrophage polarization in vivo. However, native OMVs showed low yield and limited immunogenicity. To overcome these limitations, we engineered a recombinant B. abortus strain Bru-M3, by deleting the eipB, per, and wadC gene

SUBMITTER: Huang Y 

PROVIDER: S-EPMC12639870 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature