Mutation in the knockdown resistance gene and population genetic structure in Culex tritaeniorhynchus from Guizhou Province.

ABSTRACT:

Background

Culex tritaeniorhynchus is the main vector of Japanese encephalitis virus. However, there is a gap in current research on the knockdown resistance gene (kdr) and population genetic structure of Culex tritaeniorhynchus in Guizhou Province, China.

Methods

We collected 365 Culex tritaeniorhynchus mosquitoes from ten geographic populations in Guizhou Province in 2023-2024 and analyzed the genetic diversity of the kdr gene with mutation at locus 1014 and the mitochondrial DNA-cytochrome c oxidase subunit I (mtDNA-COI) gene by polymerase chain reaction (PCR) amplification and sequencing. Haplotype diversity and nucleotide diversity were also analyzed, and a haplotype network diagram and phylogenetic tree were constructed.

Results

Only the L1014F mutation (TTA → T

SUBMITTER: Li W 

PROVIDER: S-EPMC12639896 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature