Vincristine Treatment Protects Against Podocyte Damage in Focal Segmental Glomerulosclerosis.

ABSTRACT:

Introduction

Focal segmental glomerulosclerosis (FSGS) is associated with podocyte damage resulting in cytoskeletal alterations leading to foot process effacement. Vincristine is a chemoprotective drug which alters cytoskeletal microtubules and has been used clinically to reverse FSGS. However, the mechanisms underlying the beneficial effect of vincristine are not understood.

Methods

Immortalized human podocytes were exposed to serum obtained from an adult index patient with FSGS before, during, and after vincristine treatment. We examined the podocyte transcriptome by RNA-sequencing alongside cytoskeletal structure and filtration barrier integrity using a glomerulus-on-a-chip (GOAC) model.

Results

Podocytes exposed to serum from the index patient with FSGS during or

SUBMITTER: Mason WJ 

PROVIDER: S-EPMC12640030 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature