NMR-guided rational exploration of co-factors in boosting the <i>Pfu</i> DNA polymerase.

ABSTRACT: With rapid developments of emerging technologies like synthetic biology, the demand for DNA polymerases with superior activities including higher thermostability and processivity has increased significantly. Thus, rational optimization of the performance of DNA polymerase is of great interest. Nuclear magnetic resonance (NMR) spectroscopy is a powerful technique used for studying protein structure and dynamics. It provides the atomic resolution information of enzymes under their functional solution environment to reveal the active sites (hot spots) of the enzyme, which could be further used for optimizing the performance of enzymes. In our previous work, we identified hot spot residues of Pyrococcus furiosus DNA polymerase (Pfu pol). We aim to employ these binding hot spots t

SUBMITTER: Chen Y 

PROVIDER: S-EPMC12640072 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature