The Chorioallantoic Membrane as a Platform for Developing Vascularized Cell Macroencapsulation Devices.

ABSTRACT:

Background

Cell macroencapsulation devices (CMD) offer a promising solution for organ function replacement by shielding implanted cells from the host immune system while allowing the exchange of nutrients and waste products. Developing efficient CMD necessitates optimizing vascular integration, membrane permeability, and cellular functionality using robust preclinical models. In this study, we adapted the chick chorioallantoic membrane (CAM) model to develop and evaluate CMD.

Methods

Semipermeable membranes were integrated into the CAM, with vascularization modulated through growth factors and extracellular matrix manipulation. Human kidney tubular epithelial cells were cultured on these vascularized membranes to assess cell viability, polarization, and functionality, includ

SUBMITTER: Bernardi MDL 

PROVIDER: S-EPMC12640263 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature