Amplysis: an R package for microbial composition and diversity analysis using 16S rRNA amplicon data.

ABSTRACT: The downstream analysis of 16S rRNA sequencing data remains a significant challenge for researchers lacking extensive bioinformatics expertise, often requiring proficiency in diverse tools and methodologies. To address this, we present amplysis, an R package designed to streamline the analysis and visualization of 16S rRNA amplicon sequencing data through an intuitive, code-light workflow. amplysis integrates data importing, processing, statistical analysis, and visualization into a unified framework. Key features include data normalization, microbial composition profiling, alpha/beta diversity analysis, ordination methods (e.g. Principal Component Analysis), and publication-ready visualization tools. The package's utility was demonstrated through three case studies, one of which analyzed

SUBMITTER: Su Z 

PROVIDER: S-EPMC12640542 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature