A rapid and visual dual LAMP-LFD assay for on-site simultaneous detection of influenza A virus (H1N1) and respiratory syncytial virus (RSV).

ABSTRACT: Respiratory tract infections caused by influenza A virus (IAV) and respiratory syncytial virus (RSV) are a major global health burden. To overcome the limitations of existing diagnostic techniques for timely detection (point-of-care testing), this study developed a dual rapid visual detection method based on loop-mediated isothermal amplification (LAMP) and lateral flow device (LFD) technologies for the simultaneous detection of H1N1 influenza virus and RSV. This method uses a dual-labeled probe system (H1N1: digoxigenin/biotin; RSV: 6-carboxyfluorescein/biotin) combined with a two-color latex microsphere signal system that enables the intuitive visual interpretation of multiple detection results. Compared with traditional nucleic acid detection, the entire detection process was completed

SUBMITTER: Zhang A 

PROVIDER: S-EPMC12640905 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature