Serum-soluble SDC1 in septic patients is rich in heparan sulfate, which affects the ELISA quantification values.

ABSTRACT:

Background

Soluble syndecan (sSDC) 1, which is released into the blood by shedding of the SDC1 ectodomain, is a potent biomarker of various inflammatory diseases and cancers. However, the characteristics of serum sSDC1 and methods for its quantification have not been thoroughly investigated. We investigated the properties of sSDC1 in the serum of patients with sepsis and their effects on measurement values.

Methods

Serum sSDC1 levels in patients with and without sepsis were determined using dot blot analysis and enzyme-linked immunosorbent assay (ELISA). The molecular characteristics of serum sSDC1 were evaluated using western blotting and immunoprecipitation.

Results

Serum sSDC1 ELISA values were more clearly able to distinguish between septic and non-septic patients

SUBMITTER: Akahane S 

PROVIDER: S-EPMC12641194 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature