Simultaneous Dual-Color Stimulated Raman Scattering Biomolecular Imaging with Tailored Supercontinuum Generation and Phase-Sensitive Detection.

ABSTRACT: We report on the development of a unique dual-color SRS (DC-SRS) microscopy with supercontinuum generation and phase-sensitive detection for simultaneous Raman imaging of vibrational bonds of tissue and cells in both high-wavenumber (2800-3200 cm^-1) and cell-silent regions (1800-2800 cm^-1). With the fine tuning of tailored supercontinuum generation of an 823 nm zero-dispersion photonic crystal fiber, we can obtain intense laser wavelength emissions (700 to 900 nm), serving as two separate pump beams (e.g., 800 and 850 nm) in combination with Stokes beam (1040 nm) for DC-SRS imaging. We introduced the 1/2pi phase-shifted scheme on the Stoke beam to achieve the simultaneous DC-SRS imaging under a single photodiode detection with a 2-channel lock-in amplifier. We have pr

SUBMITTER: Xin L 

PROVIDER: S-EPMC12641339 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature