Distinct intracellular spatiotemporal expression of Calmodulin genes underlies functional diversity of Calmodulin-dependent signalling in cardiac myocytes.

ABSTRACT:

Aims

This study aims to resolve the mechanisms underlying Calmodulin (CaM)'s signalling diversity by investigating whether the three CaM genes-Calm1, Calm2, and Calm3-play distinct or redundant roles in cardiac myocytes, focusing on their spatial mRNA localization and interactions with key targets.

Methods and results

We utilized single-molecule mRNA detection and three-dimensional imaging to map the spatial distribution of Calm1, Calm2, and Calm3 mRNAs within ventricular myocytes. These mRNAs were found to be consistently positioned within specific cellular zones, overlapping with their target mRNAs and forming region-specific transcript conjunctions. This spatial organization aligns with two distinct protein synthesis pathways: centralized synthesis near the nucleus for pr

SUBMITTER: Bogdanov V 

PROVIDER: S-EPMC12641389 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature