Tea Plant miR5368-p5 Negatively Regulates Resistance Against Lasiodiplodia theobromae Through Targeting the CsWRKY57-CsLRR-RLK Module.

ABSTRACT: Lasiodiplodia theobromae can cause severe diseases, including leaf spot, leaf necrosis and stem canker in tea plants, leading to substantial losses in both tea leaf production and quality. However, the mechanisms underlying host resistance remain poorly understood. In this study, we identified CsWRKY57 as a nucleus-localised transcription factor whose expression dynamically responds to L. theobromae infection. Transient overexpression and antisense oligonucleotide (AsODN)-mediated silencing in tea leaves, along with stable overexpression in transgenic Nicotiana benthamiana leaves, demonstrated that CsWRKY57 enhanced resistance to L. theobromae. Yeast two-hybrid and bimolecular fluorescence complementation assays revealed that CsWRKY57 interacts with CsTIFY5A in the nucleus, further confirm

SUBMITTER: Wang B 

PROVIDER: S-EPMC12641446 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature