Monosaccharide Binding to Synthetic Carbohydrate Receptor Microarrays.

ABSTRACT: A glycan detection platform, comprised of synthetic carbohydrate receptors (SCRs) immobilized onto polymer brushes, was prepared. SCR043, an alkene-containing SCR, was incorporated into grafted-from polymer brushes using hypersurface photolithography, resulting in microarrays of SCR043-functionalized polymer brushes, where brush height (h) and SCR grafting density (Γ) are controlled precisely at each feature in the array. The influence of h and Γ on the binding to five fluorescently labeled monosaccharidesα-glucose (α-Gluc-FL), α-galactose (α-Gal-FL), α-mannose (α-Man-FL), β-glucose (β-Gluc-FL), and β-galactose (β-Gal-FL)in aqueous buffer was investigated using fluorescence microscopy. These experiments provided 9072 d

SUBMITTER: Shlain MA 

PROVIDER: S-EPMC12641478 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature