Quantifying the liquid-liquid transition in cold water/glycerol mixtures by intermolecular hyperfine relaxation-induced dipolar modulation enhancement (ih-RIDME).

ABSTRACT: Water/glycerol mixtures are commonly used for experiments with biomacromolecules at cryogenic temperatures due to their vitrification properties. Above the glass transition temperature, they undergo liquid-liquid phase separation. Using a novel EPR technique called intermolecular hyperfine relaxation-induced dipolar modulation enhancement (ih-RIDME), we quantified the molar composition in frozen water/glycerol mixtures with one or the other component deuterated after the phase transition. Our experiments reveal a nearly equal phase composition regardless of the proton/deuterium isotope balance. With the new ih-RIDME data, we can also revisit the previously reported body of glass transition data for these mixtures and build a consistent picture of water/glycerol freezing and phase transitio

SUBMITTER: Kuzin S 

PROVIDER: S-EPMC12641492 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature