Astrocytic Connexin43 Channels Are Essential for Breathing Pattern Stabilization in the preBotzinger Complex.

ABSTRACT:

Objective

Astrocytes within the preBötzinger complex (preBötC) critically regulate respiratory rhythmogenesis and pattern formation. However, the molecular mechanisms underlying their contributions remain poorly understood. This study aims to investigate whether connexin 43 (Cx43) channels, a prominent subtype of connexin proteins expressed in preBötC astrocytes, are essential for stabilizing breathing patterns.

Methods

We employed a multidisciplinary approach, integrating whole-body plethysmography, in vivo fiber photometry, phrenic nerve discharge (PND) recordings, photostimulation, RNAscope fluorescence in situ hybridization, and RNA sequencing to elucidate the functional role of Cx43 channels in respiratory regulation.

Results

Elevated activation levels of preBötC

SUBMITTER: Zhao X 

PROVIDER: S-EPMC12641571 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature