Establishing an Ex Vivo Culture Model of Human Proximal Airway Tissue.

ABSTRACT:

Background

Developing clinically relevant experimental models of the human airway can significantly advance our understanding of the mechanisms underlying airway diseases and aid in translating potential therapies to clinical settings. The aim of this study is to establish an ex vivo human airway tissue culture model.

Methods

Human donor airway tissues were obtained from clinical cases of lung transplantation. Our established method is based on the concept of scavenging metabolic activity and controlling bacterial growth and includes increased media volume, frequent media exchange, and antifungal additives to efficiently maintain the homeostatic culture environment. After a 3-day culture period, the airway was investigated, and its viability and function were compared with a

SUBMITTER: Atale N 

PROVIDER: S-EPMC12641672 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature