Measuring concentration and diffusivity within biomolecular condensates using calibration-free scanning fluorescence correlation spectroscopy.

ABSTRACT: There is a growing consensus that cells can regulate biochemical activity through membrane-less organelles, also known as biomolecular condensates. Unfortunately, the mechanisms underlying the interplay between phase separation and biochemical reactions are still unclear. Since biochemical reactions depend strongly on the local concentrations and diffusivities of molecules in the dense phase, accurately characterizing these parameters is essential for understanding biochemical regulation within phase-separated condensates. Fluorescence correlation spectroscopies can measure these properties but are limited by their need for calibration standards. Here, we present a calibration-free method based on temporal line scan fluorescence correlation spectroscopy and sinusoidal scan fluorescence cor

SUBMITTER: Mathur P 

PROVIDER: S-EPMC12641978 | biostudies-literature | 2026 Jan

REPOSITORIES: biostudies-literature