Differentiating infected from vaccinated animals: Validation and application of NH-AGID assay in heifers immunized with Brucella abortus strain A19-∆VirB12.

ABSTRACT: Differentiating infected from vaccinated animals is crucial for clinical diagnosis in brucellosis control. The objective of this study was to assess the performance of native hapten agar gel immuno-diffusion(NH-AGID) in differential identification of infected heifers from vaccined with strain A19-∆VirB12 and identifying shedding animals at the serologic level. 30 heifers were vaccined with A19-∆VirB12, and corresponding serum and vaginal swab samples were collected at predetermined intervals. Serological assessments were conducted using the Rose-bengal test, serum agglutination test, competitive enzyme-linked immunosorbent assay and NH-AGID. Brucella-positive sera were further distinguished using differential identification ELISA and NH-AGID. Vaginal swab DNA was tested using

SUBMITTER: Ye F 

PROVIDER: S-EPMC12642129 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature