Axolotl-derived decellularized skin ECM as a pro-regenerative scaffold for attenuating fibrotic wound healing.

ABSTRACT: Conventional wound healing in mammals often results in fibrosis, leading to permanent scarring and compromised skin function. Inspired by the remarkable regenerative capacity of axolotls, we developed an axolotl skin decellularized extracellular matrix (A-dECM) as a pro-regenerative scaffold for mammalian wound healing. A-dECM retained its native ECM composition, including a glycosaminoglycan-rich and collagen-dense profile, while effectively eliminating cellular and immunogenic components, as confirmed by histological staining and DNA quantification. Whole scanning electron microscopy revealed a highly organized ultrastructure in intact A-dECM. To improve biocompatibility, potentially cytotoxic ∼40 kDa protein fractions were removed from A-dECM via size-exclusion chromatography, and the f

SUBMITTER: Wang MH 

PROVIDER: S-EPMC12642132 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature