A novel algorithm- specific loci screening accelerates the establishment of molecular quantification of Glycyrrhiza glabra, G. uralensis, and G. inflata.

ABSTRACT:

Objective

This study aims to establish a specialized molecular method for distinguishing different licorice and quantifying target species accurately from mixtures.

Methods

A rapid specific loci screening (SLS) algorithm was developed in this study, and used the chloroplast genome sequences were used to screen for variable loci in the Glycyrrhiza glabra (GG), G. uralensis (GU), and G. inflata (GI). Each locus has been validated by polymerase chain reaction and pyrosequencing. The selected loci were analyzed by Herb molecular quantification (Herb-Q) assay to complete the quantitative methodology verification and establish a quantitative detection system for homemade licorice mixtures and one of the patent medicines-Liuyi San.

Results

Outstanding performance was observe

SUBMITTER: Pei Y 

PROVIDER: S-EPMC12642167 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature