A novel protocol for protoplast isolation, transfection, and culture in Cannabis sativa L.

ABSTRACT:

Background

Protoplasts are a valuable tool for studying gene expression and applying genome editing techniques. Given the high medicinal and industrial potential of Cannabis sativa L., developing an efficient protoplast-to-plant regeneration protocol is highly desirable. Due to its recalcitrant nature, a complete plant regeneration from cannabis protoplasts has not yet been achieved.

Results

This study details a robust protocol for cannabis protoplast isolation, purification, transient transfection, and culture, additionally reporting somatic embryo-like structures derived from protoplast-derived callus. We demonstrated that the age of donor material, the composition of the enzyme solution, and the duration of enzymolysis are crucial for efficient protoplast isolation. Proto

SUBMITTER: Stelmach-Wityk K 

PROVIDER: S-EPMC12642208 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature