Development and application of a PCR-RFLP assay revealing widespread distribution of the pyrethroid resistance-associated VGSC V1016G mutation in Aedes albopictus from Guangyuan City, Sichuan Province of China.

ABSTRACT:

Background

Aedes albopictus is a primary vector of multiple arboviruses, including dengue, chikungunya, yellow fever, and Zika virus. Its control relies heavily on pyrethroid insecticides. The V1016G mutation in the voltage-gated sodium channel (VGSC) is a well-documented mechanism conferring pyrethroid resistance in Ae. albopictus, which directly challenges the efficacy of pyrethroid-based control. Understanding of the status of insecticide resistance will offer insights to inform evidence-based vector management. However, current phenotypic monitoring is laborious and time-consuming, highlighting the need for rapid and reliable genotyping tools.

Methods

To detect the V1016G mutation, we developed a polymerase chain reaction-restriction fragment length polymorphism (PCR-RFL

SUBMITTER: Lu X 

PROVIDER: S-EPMC12642244 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature