ICAM-5: A Novel Marker for Neuronally Derived Extracellular Vesicles.

ABSTRACT: Current methods for isolating neuronal-derived extracellular vesicles (NDEVs) from human biofluids lack specificity. In addition, some of the reported markers for NDEV isolation are present as soluble proteins instead of extracellular vesicle (EV) associated proteins. To address the research gap, this study aimed to identify a novel marker for NDEV isolation that is NDEV associate and highly specific to the central nervous system. To achieve this, human cortical neurons were used for isolation of EVs invitro. Mass spectroscopy was performed to screen for potential EV surface markers. This analysis yielded 63 brain specific proteins among which ICAM-5 was selected for further validations in human serum and cerebrospinal fluid (CSF) samples. Our analysis shows that ICAM-5 is present o

SUBMITTER: Deleon P 

PROVIDER: S-EPMC12642397 | biostudies-literature | 2025 Nov

REPOSITORIES: biostudies-literature